#WCE2014 - Evaluation of kidney function post-irreversible electroporation in comparison to partial nephrectomy and radiofrequency ablation in a porcine solitary kidney model - Interview

TAIPEI, TAIWAN (UroToday.com) - Introduction and Objectives: Irreversible electroporation (IRE) uses short pulses of DC electric current to create irreversible pores in the cell membrane, thereby causing cell death. We conducted a prospective trial to compare kidney function and pathological changes post-IRE in a solitary kidney porcine model to partial nephrectomy and radiofrequency ablation (RFA).

wceMethods: Fifteen domesticated pigs were randomized into 3 equal groups. All pigs underwent laparoscopic nephrectomy to create the condition of a solitary kidney. On the remaining kidney either IRE, partial nephrectomy or RFA was performed on the lower third. Serum creatinine (SCr) was taken at baseline, 24 hrs, 3, 7, 14, and 28 days postoperatively. Repeated- measures ANOVA and Student’s t-test were used to compare change in mean SCr from baseline. Acute and chronic histological changes were analyzed and cellular viability was assessed using NADH staining in the IRE porcine model.

Results: In all, 10 ablations (5 IRE, 5 RFA) and 5 partial nephrectomies of the lower third of a solitary kidney were performed. All pigs survived without complications. The type of procedure did not affect change in baseline SCr significantly (p = 0.14). The change in mean SCr from baseline was not significantly different across time for any of the three groups (p > 0.05). NADH stain showed absence of viability in the necrotic areas immediately, at days 14 and 28 and viable tissue in the areas with reparative changes.

Conclusions: IRE in the porcine kidney shows comparable changes in renal function with time as compared to other treatments. Further studies are warranted to study efficacy.

Source of Funding: None


Listen to an interview with Monica S.C. Morgan, one of the authors of this study.

 

Presented by Monica S.C. Morgan,1 Asim Ozayar,1
Elena Lucas,2 Justin I. Friedlander,1
Nabeel Shakir,1 Neil Gupta,1 and Jeffrey A. Cadeddu1 at the 32nd World Congress of Endourology & SWL - September 3 - 7, 2014 - Taipei, Taiwan

1University of Texas Southwestern, Department of Urology, Dallas, TX USA

2University of Texas Southwestern, Department of Pathology, Dallas, TX USA

 

Conference Coverage
 
Recent data from conferences worldwide
  • 2020 Virtual Congress / September 19-21
  • 2020 BCAN Think Tank Virtual / August 7
  • 2020 Virtual Education Program / August 8-10
  • 2020 Annual Meeting / July 17-19 / EAU Virtual Program
  • SUO - AUA 2020 Summer Webcast / July 18
  • 2020 Annual Meeting / June 27-28 / AUA Live Virtual Program
  • 2020 Annual Meeting / May 29-31 / Virtual Scientific Program
  • 2020 Annual Meeting / February 13-15 / San Francisco, CA
  • 2019 Annual Meeting / December 4-6 / Washington, DC
  • 2019 Annual Meeting / October 3 - October 5 / Aarhus, Denmark
  • 2019 Annual Congress / September 27 - October 1 / Barcelona, Spain
  • 2019 Biennial Meeting / August 29-31 / Basel, Switzerland