State-of-the-industry video lectures by leading urology experts
Latest Videos
Featured Videos

STOCKHOLM, SWEDEN ( - A third of patients with metastatic RCC will have resistance to targeted agents, and even more patients will develop resistance over time. Concerns regarding toxicity, high cost, resistance, and genetic testing have limited the ability to offer personalized therapy. The ability to predict patient response prior to the initiation of treatment could improve patient outcomes. Dr. C. R. Mazzola and colleagues developed a patient-derived xenograft model to determine the sensitivity of each RCC patient’s tumor cells to a defined targeted therapy prior to the start of systemic treatment. The investigators developed a patient−derived xenograft model using chicken embryos that allows evaluation of the interplay between angiogenesis and tumor growth and direct drug application to tumors to evaluate their sensitivities. Seven patient-derived primary RCC cell lines were implanted into the chorioallantoic membrane (CAM) of chicken embryos to assess the effect of sunitinib on tumor take ratios.

eauThree cell lines known to be sensitive to sunitinib (XP206, XP158, XP185) and four known to be resistant to sunitinib (XP127, XP121, XP258, PF22) were utilized. A secondary cell line that is sensitive to sunitinib (786-0) was also implanted as a control. In the control group, tumor take rates were 82% in vehicle treated tumors compared to 46% in sunitinib treated tumors. Using the 7 patient-derived RCC cell lines implanted into the CAM, tumor take rates varied amongst cell lines. Overall, decreased tumor take rates were observed in sunitinib-sensitive cell lines treated with sunitinib when compared to their vehicle-treated control counterparts. Extensive angiogenesis was observed among tumor xenografts.

In conclusion, the CAM model evaluated in this pilot study demonstrated the ability to predict sensitivity to sunitinib therapy using patient derived RCC cell lines. The authors have developed a useful model for drug sensitivity evaluation that may enable clinicians to potentially individualize targeted treatments to each patient with metastatic RCC prior to the start of systemic therapy. Future studies will focus on additional drugs commonly used to treat RCC.

Presented by C. R. Mazzola at the 29th Annual European Association of Urology (EAU) Congress - April 11 - 15, 2014 - Stockholmsmässan - Stockholm, Sweden.

Western University, Dept. of Surgery, London, Ontario, Canada

Written by Jeffrey J. Tomaszewski, MD, medical writer for


Publications focusing on urological cancer treatments through original commentary & articles
Everyday Urology Volume 3 Issue 2

Everyday Urology™ - Oncology Insights

From the Editor

PCAN cover

Prostate Cancer and Prostatic Diseases

From the Editor


Newsletter subscription

Free Daily and Weekly newsletters offered by content of interest

The fields of GU Oncology and Urology are rapidly advancing. Sign up today for articles, videos, conference highlights and abstracts from peer-review publications by disease and condition delivered to your inbox and read on the go.


Upcoming educational events
October 19-23, 2018 / European Society for Medical Oncology 2018 Congress
European Society for Medical Oncology 2018 Congress
October 19-20, 2018 / Hilton New York JFK Airport Hotel
16th International Conference on Nephrology & Therapeutics
October 19-20, 2018 / Hilton New York JFK Airport Hotel
3rd Annual Kidney Congress