| Vascular Endothelial Growth Factor (VEGF) Gene Therapy Using a Non-Viral Gene Delivery System Improves Erectile Dysfunction in a Diabetic Rat Model |
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| Wednesday, 15 November 2006 | ||||
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NEW ORLEANS, LA (UroToday.com) - American Society of Reproductive Medicine (ASRM) New Orleans - 2006 Diabetes was induced and confirmed with a single injection of streptozotocin. Animals then received intracavernous injection with 10 ug plasmid DNA encoding a fusion VEGF/green fluorescent protein (GFP) complex . Fluorescence microscopy revealed expression of GFP in all treated animals. Western blot analysis confirmed expression of the GFP/VEGF fusion protein, and RT-PCR confirmed the presence of mRNA transcript within the cavernosal tissue of all treated rats and immunohistochemistry analysis of the cavernosal tissue showed an increased number of smooth muscle cells compared to the diabetic controls The authors concluded that this model achieved in-vivo transfection of VEGF into rat corpus cavernosum using a non-viral gene delivery system. Expression of the transected VEGF leads to an overall improvement of maximal intracorporeal cavernous pressure and smooth muscle content. Editorial Comments; J. N. Mills, J. Dall'era, S. Koul, J. Myers, R. B. Meacham, H. Koul. University of Colorado Health Sciences Center, Denver, CO UroToday.com Conference Reports
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